Rainbow, brown and cutthroat trout are found in East Rifle Creek, which flows through Rifle Falls. Fish up to 19 inches long have been taken, with 6- to 9- inch fish being the average size.
The CPW Rifle Falls Fish Hatchery, is upstream of Rifle Falls. It is one of the largest trout production hatcheries in Colorado. It can be reached by hiking the Bobcat Trail or by continuing up Highway 325. Free tours of the Hatchery are available during the summer.